期刊名称 protein expression and purification
期刊缩写 PROTEIN EXPRES PURIF
学科分类 生命科学、医学、化学、工程学
出版周期 月刊
审稿速度 1.66 个月 （平均）
投稿命中率 95 % （均值)
期刊主页 http://www.journals.elsevier.com/protein-expression-and-purification
ISSN 号 (printed): 1046-5928 (electronic): 1096-0279
IF 趋势 2005 2006 2007 2008 2009 2010 2011 2012
1.553 1.867 1.94 1.621 1.563 1.644 1.587 1.429
被收录情况 1. Science Citation Index
2. Science Citation Index Expanded
3. Current Contents - Life Sciences
4. BIOSIS Previews
PubSci评语 一审周期很短，相对容易，编辑只要送去外审就一般没什么问题。当然文章要有新意。编辑和审稿人都比较Nice。国人投稿比较比较大。
期刊简介 该刊主要接受以下几类研究成果：1. 研究高纯度蛋白分离新方法或显著优化后的方法的原创性论文。2. 从遗传工程资源中表达和分离蛋白的方法。3. 过表达特定蛋白的新奇或优化的分子生物学方法。4. 描述和评估蛋白表达和纯化重要方法的综述文章。
投稿注意事项 特别提醒：在线发表的彩页不计费，印刷形式出版的需要收费。Open Access形式发表论文，需要交纳3000美元Open access fee。Subscription形式发表的论文无需缴纳此费用。
一般注意事项：
Protein Expression and Purification is an international journal designed to provide biochemists, molecular biologists, and other investigators with a forum for presenting significant advances in protein isolation. The journal publishes original articles on novel or improved isolations of specific proteins from conventional and genetically engineered sources.
Protein Expression and Purification's emphasis is on the application of expression and purification procedures to the production of proteins. Findings should be novel and of general interest to scientists involved in the protein sciences. Articles should focus on new methods which are widely applicable in the field or detailed application of a method to an important protein. Articles must discuss the significance and/or application of these findings and should include a detailed description of the approaches utilized with significant characterization of a purified protein. Simple purification strategies using common affinity purification techniques without general significance are unacceptable for publication.
Types of paper
Review articles describing and evaluating important advances in the expression and purification of proteins will be featured. Such reviews are generally invited; interested authors should contact the Editor-in-Chief. Topics for original articles include but are not limited to:
The preparation of natural and recombinant proteins; Achievement of a high level of purity; Expression of active recombinant proteins; Production of mutant proteins; High-throughput purification; Improved or novel fractionation techniques for protein expression; Control of stability, solubility, and activity; Gene constructions designed to facilitate purification; Vectors and hosts; High-throughput expression; careful comparisons of several expression systems, refolding conditions or related chromatography resins.
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Conflict of interest
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Language (usage and editing services)
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Submission
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Referees
Please submit, with the manuscript, the names, addresses and e-mail addresses of three potential referees. Note that the editor retains the sole right to decide whether or not the suggested reviewers are used.
Additional Information
Protein isolation and expression procedures should be described in sufficient detail to allow the procedures to be used without extensive reference to previously published studies. Well-established procedures such as methods of protein determination and general techniques of vector construction should be cited only by literature reference, but the procedures for determining enzyme activity or for specifically quantifying non-enzymatic proteins (e.g., immunoreactivity or densitometric quantitation of protein gels) should be described in sufficient detail to allow the methods to be applied without reference to previous descriptions. For assay methods that have been reported previously, the description should be as brief as is consistent with direct application of the method; the original report of the method should be cited. For novel assay procedures, the experimental description should be complete and include evidence of specificity, sensitivity, and linearity of response with time and amount of protein.
Where appropriate, materials should have their supplier and catalog number recorded to assist replication of results.
Vector construction should be diagrammed adequately showing relevant marker genes, regulatory elements, insert and vector sizes, and key restriction enzyme sites. If indicated restriction enzyme sites are not unique in the construct, this fact must be stated. Information regarding the construction of novel vectors or recombinant molecules should be provided in sufficient detail to allow the work to be evaluated and applied. It is expected that the authors will honor the normal scientific practice of making available on request, any new strains constructed and described in the manuscript. Where appropriate, DNA sequences should be reported. Previously unpublished gene or cDNA sequences must be submitted to a public data base (e.g., GenBank/ EMBL Data Bank) prior to publication; accession numbers will be included in the published paper as a footnote.
GenBank/DNA sequence linking. Authors wishing to enable other scientists to use the accession numbers cited in their papers via links to these sources should type this information in the following manner:
For each and every accession number cited in an article, authors should type the accession number in bold, underlined text . Letters in the accession number should always be capitalized (see example below). This combination of letters and format will enable the typesetter to recognize the relevant texts as accession numbers and add the required link to GenBank's sequences.
Example: GenBank accession nos. AI631510 , AI631511 , AI632198 , and BF223228, a B-cell tumor from a chronic lymphatic leukemia (GenBank accession no. BE675048 ), and a T-cell lymphoma (GenBank accession no.AA361117 ).
Authors are encouraged to check accession numbers used very carefully. An error in a letter or number can result in a dead link. In the final version of the printed article, the accession number text will not appear bold or underlined. In the final version of the electronic copy, the accession number text will be linked to the appropriate source in the NCBI databases, enabling readers to go directly to that source from the article.
Use of word processing software
It is important that the file be saved in the native format of the wordprocessor used. The text should be in single-column format. Keep the layout of the text as simple as possible. Most formatting codes will be removed and replaced on processing the article. In particular, do not use the wordprocessor's options to justify text or to hyphenate words. However, do use bold face, italics, subscripts, superscripts etc. Do not embed "graphically designed" equations or tables, but prepare these using the wordprocessor's facility. When preparing tables, if you are using a table grid, use only one grid for each individual table and not a grid for each row. If no grid is used, use tabs, not spaces, to align columns. The electronic text should be prepared in a way very similar to that of conventional manuscripts (see also the Guide to Publishing with Elsevier:http://www.elsevier.com/guidepublication). Do not import the figures into the text file but, instead, indicate their approximate locations directly in the electronic text and on the manuscript. See also the section on Electronic illustrations.
To avoid unnecessary errors you are strongly advised to use the "spell-check" and "grammar-check" functions of your wordprocessor. Do not capitalize chemical names (e.g., urea) and do capitalize trade names (e.g., Sepharose).
Article structure
Introduction
State the objectives of the work and provide an adequate background, avoiding a detailed literature survey or a summary of the results.
Material and Methods
Provide sufficient detail to allow the work to be reproduced, with details of supplier and catalogue number when appropriate. Methods already published should be indicated by a reference: only relevant modifications should be described.
Results
Results should be clear and concise.
Discussion
This should explore the significance of the results of the work, not repeat them. A combined Results and Discussion section is often appropriate. Avoid extensive citations and discussion of published literature.
Conclusions
The main conclusions of the study may be presented in a short Conclusions section, which may stand alone or form a subsection of a Discussion or Results and Discussion section.
Appendices
If there is more than one appendix, they should be identified as A, B, etc. Formulae and equations in appendices should be given separate numbering: Eq. (A.1), Eq. (A.2), etc.; in a subsequent appendix, Eq. (B.1) and so on. Similarly for tables and figures: Table A.1; Fig. A.1, etc.
Essential title page information
• Title. Concise and informative. Titles are often used in information-retrieval systems. Avoid abbreviations and formulae where possible.
• Author names and affiliations. Where the family name may be ambiguous (e.g., a double name), please indicate this clearly. Present the authors' affiliation addresses (where the actual work was done) below the names. Indicate all affiliations with a lower-case superscript letter immediately after the author's name and in front of the appropriate address. Provide the full postal address of each affiliation, including the country name and, if available, the e-mail address of each author.
• Corresponding author. Clearly indicate who will handle correspondence at all stages of refereeing and publication, also post-publication. Ensure that phone numbers (with country and area code) are provided in addition to the e-mail address and the complete postal address. Contact details must be kept up to date by the corresponding author.
• Present/permanent address. If an author has moved since the work described in the article was done, or was visiting at the time, a 'Present address' (or 'Permanent address') may be indicated as a footnote to that author's name. The address at which the author actually did the work must be retained as the main, affiliation address. Superscript Arabic numerals are used for such footnotes.
Abstract
A concise and factual abstract is required. The abstract should state briefly the purpose of the research, the principal results and major conclusions. An abstract is often presented separately from the article, so it must be able to stand alone. For this reason, References should be avoided, but if essential, then cite the author(s) and year(s). Also, non-standard or uncommon abbreviations should be avoided, but if essential they must be defined at their first mention in the abstract itself. Please indicate the host organism used for expression of a recombinant gene.
Graphical abstract
A Graphical abstract is optional and should summarize the contents of the article in a concise, pictorial form designed to capture the attention of a wide readership online. Authors must provide images that clearly represent the work described in the article. Graphical abstracts should be submitted as a separate file in the online submission system. Image size: Please provide an image with a minimum of 531 × 1328 pixels (h × w) or proportionally more. The image should be readable at a size of 5 × 13 cm using a regular screen resolution of 96 dpi. Preferred file types: TIFF, EPS, PDF or MS Office files. See http://www.elsevier.com/graphicalabstracts for examples.
Authors can make use of Elsevier's Illustration and Enhancement service to ensure the best presentation of their images also in accordance with all technical requirements: Illustration Service.
Highlights
Highlights are mandatory for this journal. They consist of a short collection of bullet points that convey the core findings of the article and should be submitted in a separate file in the online submission system. Please use 'Highlights' in the file name and include 3 to 5 bullet points (maximum 85 characters, including spaces, per bullet point). See http://www.elsevier.com/highlights for examples.
Keywords
Immediately after the abstract, provide a maximum of 6 keywords, using American spelling and avoiding general and plural terms and multiple concepts (avoid, for example, 'and', 'of'). Be sparing with abbreviations: only abbreviations firmly established in the field may be eligible. These keywords will be used for indexing purposes.
Abbreviations
Define abbreviations that are not standard in this field in a footnote to be placed on the first page of the article. Such abbreviations that are unavoidable in the abstract must be defined at their first mention there, as well as in the footnote. Ensure consistency of abbreviations throughout the article.
Acknowledgements
Collate acknowledgements in a separate section at the end of the article before the references and do not, therefore, include them on the title page, as a footnote to the title or otherwise. List here those individuals who provided help during the research (e.g., providing language help, writing assistance or proof reading the article, provided strains or materials, etc.).
Database linking
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Footnotes
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Table footnotes
Indicate each footnote in a table with a superscript lowercase letter.
Artwork
Electronic artwork
General points
• Make sure you use uniform lettering and sizing of your original artwork.
• Embed the used fonts if the application provides that option.
• Aim to use the following fonts in your illustrations: Arial, Courier, Times New Roman, Symbol, or use fonts that look similar.
• Number the illustrations according to their sequence in the text.
• Use a logical naming convention for your artwork files.
• Provide captions to illustrations separately.
• Size the illustrations close to the desired dimensions of the printed version.
• Submit each illustration as a separate file.
A detailed guide on electronic artwork is available on our website:
http://www.elsevier.com/artworkinstructions
You are urged to visit this site; some excerpts from the detailed information are given here.
Formats
If your electronic artwork is created in a Microsoft Office application (Word, PowerPoint, Excel) then please supply 'as is' in the native document format.
Regardless of the application used other than Microsoft Office, when your electronic artwork is finalized, please 'Save as' or convert the images to one of the following formats (note the resolution requirements for line drawings, halftones, and line/halftone combinations given below):
EPS (or PDF): Vector drawings, embed all used fonts.
TIFF (or JPEG): Color or grayscale photographs (halftones), keep to a minimum of 300 dpi.
TIFF (or JPEG): Bitmapped (pure black & white pixels) line drawings, keep to a minimum of 1000 dpi.
TIFF (or JPEG): Combinations bitmapped line/half-tone (color or grayscale), keep to a minimum of 500 dpi.
Please do not:
• Supply files that are optimized for screen use (e.g., GIF, BMP, PICT, WPG); these typically have a low number of pixels and limited set of colors;
• Supply files that are too low in resolution;
• Submit graphics that are disproportionately large for the content.
Color artwork
Please make sure that artwork files are in an acceptable format (TIFF (or JPEG), EPS (or PDF), or MS Office files) and with the correct resolution. If, together with your accepted article, you submit usable color figures then Elsevier will ensure, at no additional charge, that these figures will appear in color on the Web (e.g., ScienceDirect and other sites) regardless of whether or not these illustrations are reproduced in color in the printed version. For color reproduction in print, you will receive information regarding the costs from Elsevier after receipt of your accepted article. Please indicate your preference for color: in print or on the Web only. For further information on the preparation of electronic artwork, please see http://www.elsevier.com/artworkinstructions.
Please note: Because of technical complications which can arise by converting color figures to 'gray scale' (for the printed version should you not opt for color in print) please submit in addition usable black and white versions of all the color illustrations.
Figure captions
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Tables
Number tables consecutively in accordance with their appearance in the text. Place footnotes to tables below the table body and indicate them with superscript lowercase letters. Avoid vertical rules. Be sparing in the use of tables and ensure that the data presented in tables do not duplicate results described elsewhere in the article.
References
Citation in text
Please ensure that every reference cited in the text is also present in the reference list (and vice versa). Any references cited in the abstract must be given in full. Unpublished results and personal communications are not recommended in the reference list, but may be mentioned in the text. If these references are included in the reference list they should follow the standard reference style of the journal and should include a substitution of the publication date with either 'Unpublished results' or 'Personal communication'. Citation of a reference as 'in press' implies that the item has been accepted for publication.
Web references
As a minimum, the full URL should be given and the date when the reference was last accessed. Any further information, if known (DOI, author names, dates, reference to a source publication, etc.), should also be given. Web references can be listed separately (e.g., after the reference list) under a different heading if desired, or can be included in the reference list.
References in a special issue
Please ensure that the words 'this issue' are added to any references in the list (and any citations in the text) to other articles in the same Special Issue.
Reference management software
This journal has standard templates available in key reference management packages EndNote (http://www.endnote.com/support/enstyles.asp) and Reference Manager (http://refman.com/support/rmstyles.asp). Using plug-ins to wordprocessing packages, authors only need to select the appropriate journal template when preparing their article and the list of references and citations to these will be formatted according to the journal style which is described below.
Reference style
Text: Indicate references by number(s) in square brackets in line with the text. The actual authors can be referred to, but the reference number(s) must always be given.
Example: '..... as demonstrated [3,6]. Barnaby and Jones [8] obtained a different result ....'
List: Number the references (numbers in square brackets) in the list in the order in which they appear in the text.
Examples:
Reference to a journal publication:
[1] J. van der Geer, J.A.J. Hanraads, R.A. Lupton, The art of writing a scientific article, J. Sci. Commun. 163 (2010) 51–59.
Reference to a book:
[2] W. Strunk Jr., E.B. White, The Elements of Style, fourth ed., Longman, New York, 2000.
Reference to a chapter in an edited book:
[3] G.R. Mettam, L.B. Adams, How to prepare an electronic version of your article, in: B.S. Jones, R.Z. Smith (Eds.), Introduction to the Electronic Age, E-Publishing Inc., New York, 2009, pp. 281–304.
Journal abbreviations source
Journal names should be abbreviated according to the
List of title word abbreviations: http://www.issn.org/2-22661-LTWA-online.php.
Video data
Elsevier accepts video material and animation sequences to support and enhance your scientific research. Authors who have video or animation files that they wish to submit with their article are strongly encouraged to include links to these within the body of the article. This can be done in the same way as a figure or table by referring to the video or animation content and noting in the body text where it should be placed. All submitted files should be properly labeled so that they directly relate to the video file's content. In order to ensure that your video or animation material is directly usable, please provide the files in one of our recommended file formats with a preferred maximum size of 50 MB. Video and animation files supplied will be published online in the electronic version of your article in Elsevier Web products, including ScienceDirect: http://www.sciencedirect.com. Please supply 'stills' with your files: you can choose any frame from the video or animation or make a separate image. These will be used instead of standard icons and will personalize the link to your video data. For more detailed instructions please visit our video instruction pages at http://www.elsevier.com/artworkinstructions. Note: since video and animation cannot be embedded in the print version of the journal, please provide text for both the electronic and the print version for the portions of the article that refer to this content.
AudioSlides
The journal encourages authors to create an AudioSlides presentation with their published article. AudioSlides are brief, webinar-style presentations that are shown next to the online article on ScienceDirect. This gives authors the opportunity to summarize their research in their own words and to help readers understand what the paper is about. More information and examples are available at http://www.elsevier.com/audioslides. Authors of this journal will automatically receive an invitation e-mail to create an AudioSlides presentation after acceptance of their paper.
Supplementary data
Elsevier accepts electronic supplementary material to support and enhance your scientific research. Supplementary files offer the author additional possibilities to publish supporting applications, high-resolution images, background datasets, sound clips and more. Supplementary files supplied will be published online alongside the electronic version of your article in Elsevier Web products, including ScienceDirect:http://www.sciencedirect.com. In order to ensure that your submitted material is directly usable, please provide the data in one of our recommended file formats. Authors should submit the material in electronic format together with the article and supply a concise and descriptive caption for each file. For more detailed instructions please visit our artwork instruction pages at http://www.elsevier.com/artworkinstructions.
Supplementary material captions
Each supplementary material file should have a short caption which will be placed at the bottom of the article, where it can assist the reader and also be used by search engines.
Submission checklist
The following list will be useful during the final checking of an article prior to sending it to the journal for review. Please consult this Guide for Authors for further details of any item.
Ensure that the following items are present:
One author has been designated as the corresponding author with contact details:
• E-mail address
• Full postal address
• Phone numbers
All necessary files have been uploaded, and contain:
• Keywords
• All figure captions
• All tables (including title, description, footnotes)
Further considerations
• Manuscript has been 'spell-checked' and 'grammar-checked'
• References are in the correct format for this journal
• All references mentioned in the Reference list are cited in the text, and vice versa
• Permission has been obtained for use of copyrighted material from other sources (including the Web)
• Color figures are clearly marked as being intended for color reproduction on the Web (free of charge) and in print, or to be reproduced in color on the Web (free of charge) and in black-and-white in print
• If only color on the Web is required, black-and-white versions of the figures are also supplied for printing purposes
For any further information please visit our customer support site at http://support.elsevier.com.
Please ensure that each supplementary file has a caption in your article to enable search engine findability.
Additional information preparation
For articles reporting the isolation of specific enzymes, the Results section should include a table listing each step of the procedure, and for that step the total protein, total enzyme units, and the calculated specific activity, percentage yield, and approximate purity. For proteins without catalytic activity, an analogous table should be included indicating for each step the total protein, the amount of specific protein of interest, and the calculated fold enrichment and percentage yield. Do not report values to more than 2-3 significant figures and percentages to more than 2 significant figures. For additional guidance see "Burgess, R.R. Preparing a purification summary table. Meth. Enzymol. 463 (2009) 29-34". Figures showing chromatographic elution profiles are appropriate if the separation being illustrated is complex and cannot adequately be described in the text.
Authors should indicate which aspects of the procedure have been optimized or are particularly critical to the success of the procedure.
Articles describing the isolation of specific proteins should report sufficient catalytic or physical properties of the purified protein to establish its identity. This information (M r, subunits, pI, pH optimum, etc.) may best be presented in tabular form. Where isoforms are known or expected, the specific isoform purified should be identified. In general, only procedures leading to highly purified proteins will be considered for publication. Evidence of purity should therefore be provided and may include protein gels, immunological studies, or, for enzymes, comparison of the final specific activity with the specific activity obtained in previous studies.
Names of chemical or organic substances should follow the recommendations of the IUPAC-IUBMB Joint Combined Commission on Biochemical Nomenclature (JCBN).
Authors should draw attention to any particular chemical or biological hazards that may be involved in carrying out the experiments described. Any relevant safety precautions should be described; if an accepted code of practice has been followed, a reference to the relevant standards should be given. Also, if animals are used in the experiments, there should be a statement that the procedures followed institutional animal use and welfare guidelines. Since it is evident that the value of a purification or expression method is entirely dependent on the availability of all materials, sources of critical reagents and instruments must be clearly identified.
The journal accepts reports of studies involving recombinant DNA molecules, constructed in vitro and subsequently inserted into cells, with the understanding that the authors have adhered to appropriate NIH guidelines and/or other pertinent regulations. Procedures requiring special facilities or precautions should be identified and described in detail.
Use of the Digital Object Identifier
The Digital Object Identifier (DOI) may be used to cite and link to electronic documents. The DOI consists of a unique alpha-numeric character string which is assigned to a document by the publisher upon the initial electronic publication. The assigned DOI never changes. Therefore, it is an ideal medium for citing a document, particularly 'Articles in press' because they have not yet received their full bibliographic information. Example of a correctly given DOI (in URL format; here an article in the journal Physics Letters B):
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